Multicolor Flow Cytometry Panel Design

Constructing multicolor panels for flow cytometry can be a challenging task.

Multicolor flow cytometry panel design.

Keep adding until you have all the markers you want. To find more information check your cytometer manual view our instrument guide page or check with your local flow core facility. Know your instrument and the fluorophores that you can use. When designing a multicolor flow cytometry panel there are several key points to consider.

Build multicolor flow cytometry panels in just a few simple steps start your design by first selecting your instrument from the drop down menu or create a customized instrument to build a personalized panel select the target and species you want to identify. Flow cytometry multicolour panel design well planned and executed assays can allow the detection and monitoring of multiple parameters simultaneously in complex samples. However increasing the number of antigens and fluorochromes also increases the complexity of the experimental design. Our comprehensive portfolio of conjugated antibodies is designed to help characterize cells through surface intracellular or secreted markers.

Such multicolour assays can produce an enormous amount of data from precious or limited samples. Panel design and multicolor flow cytometry information advances in both flow cytometry reagents and instrumentation allow researchers to run increasingly complex multicolor experiments. Use a tool like the molecular probes fluorescence spectraviewer to visualize the spectral overlap of fluorophores. Know the configuration of the instrument being used laser and filters before you begin.

Multicolor flow cytometry panel design a quick and easy guide to help you build multicolor panels for flow cytometry. Flow cytometry is a powerful tool for identifying and analyzing multiple antigens simultaneously. Follow these guidelines and recommendations to achieve optimized panels.